To test this, two crews of “Biospherians” were sealed inside the structure, tasked with maintaining the ecosystems while growing their own food and processing their own waste without outside help. Declining oxygen levels, food production challenges, and interpersonal conflicts complicated the first mission, which ran from 1991 to 1993. The second mission (1994) prematurely ended after two members of the first crew broke containment from the outside, alleging that new facility management would endanger the new crew.
Since then, rather than being confined within it, scientists have utilized B2’s facilities to model climate change, uncover the mechanisms behind biodiversity loss, and develop techniques for enhancing biome sustainability.