Tardigrades

Tardigrade samples can be collected by gathering moss and submerging it in tap water for 24 hours. The moss should then be cut and drained with a mesh sieve, and the water should be collected. Samples are observable in the water between 10x and 40x magnifications.

Before being renamed for their movements, German pastor Johann August Ephraim Goeze called them Kleiner Wasserbär—little water bears—in the first published observations of the creatures in 1773. He noted their voracious appetite made them seem like bears under a microscope.

Freshwater-bound eutardigrades live on mosses and lichens and are distinguished by variations in the shape and number of claws at the end of their legs. Marine heterotardigrades, which live on algae and deep-sea sediments, have been observed to possess feathers, inflatable appendages, and flagpole-like tails.

As of 2024, three tardigrade fossils have been discovered, all preserved in amber. Analysis suggests different species of the organism existed over 500 million years ago, when marine heterotardigrades and freshwater terrestrial eutardigrades diverged.

Rather than having a circulatory system, oxygen is directly absorbed through their skin and spreads through a fluid called hemolymph as tardigrades crawl. When molting to grow, they shed their outer skin and stylets—sharp mouth parts used to pierce prey—which are regrown in their salivary glands.

Those fired from guns at speeds as high as 825 meters per second (1,845 mph) could be resuscitated after impact. Shock pressure from greater speeds tore apart the samples, suggesting they could not survive asteroid impacts if brought to Earth from outer space.

A 2007 mission launched several species into low-Earth orbit, exposing them to microgravity in temperature-controlled modules. Survivability rates were high for microgravity exposure alone, but plummeted when combined with exposure to the full UV spectrum and the vacuum of space.

Although often considered indestructible, scientists observed these creatures could endure temperatures near 150°C for only a few minutes in their tun state. Outside of this state, just half the samples in a Danish study survived a temperature of 37.1°C (98°F) for 24 hours.

Researchers have attempted to create more drought-resistant crops by introducing proteins that help protect tardigrades in their dehydrated tun state. The proteins’ ability to stabilize molecular degradation may allow scientists to develop refrigeration-free storage for vaccines, insulin, and blood.

A key component of a tardigrade’s biochemical toolkit, the damage suppressor protein is produced and binds to DNA molecules when the animal enters its dormant tun state. Dsup has provided a 50% reduction in double-strand DNA breaks in mice after exposure to X-rays.