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Scientists determined that tectonic plates moved the ocean floor by observing symmetric patterns of magnetic stripes on opposite sides of underwater volcano systems, which could only result from seafloor spreading.

Findings
Additional insights we found via Smithsonian National Museum of Natural History
At these mid-ocean ridges, erupted material forms new oceanic crust, which pushes older ocean crust away on either side of the mountain range.
Because scientists knew that the Earth's magnetic field flipped based on evidence in surface igneous rocks, whose embedded metals aligned with the field when they cooled and hardened, they could match seafloor patterns to the timeline developed from surface rocks to identify the rate of seafloor spreading and ocean floor recycling.
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